Galilei is a surname, and may refer to:
- Galileo Galilei (1564–1642), astronomer, philosopher, and physicist.
 - Vincenzo Galilei (1520–1591), composer, lutenist, and music theorist; father of Galileo
 - Michelagnolo Galilei (1575–1631), Baroque lutenist and composer, brother of Galileo
 - Alessandro Galilei (1691–1736), Florentine mathematician and architect
 
Two craters have been named for Galileo Galilei, with a different spelling:
- Galilaei (lunar crater), on the Moon
 - Galilaei (Martian crater), on Mars
 
See also
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